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Mice expressing Cre recombinase under the mouse Gfap promoter ( mGfap Cre mice) were stereotactically injected with adeno-associated virus (AAV) vectors encoding Gstm1 or non-silencing control shRNAmir into the mPFC as previously described (Figure 2A)

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The above procedure was repeated with inhibitor concentrations spanning 0.3 60 M to determine the effective concentration (EC 50 ) required to inhibit 50% NNMT activity in cultured adipocytes

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Karper JC, Ewing MM, Habets KL, de Vries MR, Peters EA, van Oeveren-Rietdijk AM, de Boer HC, Hamming JF, Kuiper J, Kandimalla ER, La Monica N, Jukema JW, Quax PH