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Description

J Cancer Res Clin Oncol 150(10):112

doi: 10.1371/journal.ppat.1007390 209 OnofrioL.CaragliaM.FacchiniG.MargheritaV.De PlacidoS.BuonerbaC

Immunofluorescence staining For most of the immunostaining experiments, cells were fixed with ice-cold methanol for 5 min at 20, washed three times with PBST (0.1% TritonX-100 in PBS) for 5 min and incubated for 30 min with 1% BSA at room temperature