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For HT-29 cells, antibodies were diluted in a permeabilization and blocking buffer containing 3% BSA, 1% saponin, and 1% Triton X-100 in PBS, and incubated overnight at RT

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Instrument control and data acquisition was achieved via LabSolutions software (Shimadzu Corporation, Kyoto, Japan)

The effect of hypothyroidism, hyperthyroidism, and their treatment on parameters of oxidative stress and antioxidant status

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