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doi: 10.1146/annurev.bi.52.070183.003431 15 HaniganMHRickettsWA

Separation and Quantification of SAM and SAH by LC-MS/MS The concentrations of SAM, SAH, and Crea from solutions prepared as described in the methods (Figure 2a) could be determined simultaneously by reverse-phased LC using isocratic solvent conditions and MS as a detection system

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A key focus should be the interaction between the HIF-1-regulated iron metabolism axis (such as transferrin receptor 1 and ferritin light chain) and ferroptosis-related molecules including GPX4 and Nrf2, to establish a dynamic model connecting hypoxiaischemia, iron dysregulation, and oligodendrocyte ferroptosis, and to identify stage-specific vulnerability across oligodendrocyte maturation