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HK-2 cells were cultured in a Dulbecco's modified Eagle's (DMEM)/F12 medium (Gibco, USA), supplemented with 10% fetal bovine serum (FBS, Biological Industries, Israel) and 1% penicillin (C0222, Beyotime Biotech Inc, China) at 37 C and 5% CO 2 in a cell culture incubator (Binder, Tuttlingen, Germany)

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Comparative GCMS based nutrients profiling of less explored legume seeds of Melilotus, Medicago, Trifolium, and Ononis analysed using chemometric tools