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Evaluation of hemorrhage, sample size, and collateral damage for five hepatic biopsy methods in dogs

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Acknowledgements The authors thank the finantial support from FAPESP, Fundao de Amparo Pesquisa do Estado de So Paulo (grant number: 2025/28627-4), CAPES, Coordenao de Aperfeioamento de Pessoal de Nvel Superior (grant number 88887.991854/2024-00, recipient JMS), and CNPq, Conselho Nacional de Desenvolvimento Cientfico e Tecnolgico (grant number 308164/2022-9, recipient ACS)

Here, we demonstrated the existence of succinylation modification on three K sites of GCLC, and their succinylation-simulating mutations nearly abolished the GCLC catalyzing activity, suggesting a pivotal role of succinylation modification in modulating GCLC enzymatic activity

This allows researchers to simulate acute, natural physiological pulses rather than continuous elevation