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The SLC38A5/SNAT5 amino acid transporter: from pathophysiology to pro-cancer roles in the tumor microenvironment

doi: 10.1016/j.arr.2018.04.004 149 ForsythCBShannonKMKordowerJHVoigtRMShaikhMJaglinJAet al

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Injection technique Proper injection technique matters for both safety and effectiveness

On the next day, targeting plasmids (sgRNA or overexpression) were co-transfected with pMD2.G (#12259, Addgene) and psPAX2 (#12260, Addgene) plasmids at 1:1:1 molar ratio using Lipofectamine 2000 (#11668019, Thermo Fisher) at 1:2.5 ratio