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While the above-cited untargeted analytical studies provided a comprehensive overview of the chemical composition of THS 2.2 aerosols and an evaluation of which constituents are present only in THS 2.2 aerosol or are more abundant than in reference cigarette smoke, they do not allow for an unequivocal conclusion on which of the observed differences are an implicit characteristic of the THS platform (i.e., the effect of heating instead of burning tobacco)

Other findings included signs of possible underlying eating disorders, insufficient levels of physical activity and several misconceptions regarding tobacco use, namely shisha and dokha

The fixed samples were then quenched with 50 mM NH 4 Cl in PBS-C/M for 10 min, followed by blocking with PBS-C/M containing 0.5% BSA, 0.5% saponin, and 0.2 mg/ml sodium azide for 30 min

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