In order to translate observed breathing patterns and smoking behavior into biological effects, we combined the small airway-on-a-chip with a smoking machine that burns cigarettes and a microrespirator that inhales and exhales small volumes of cigarette smoke and fresh air in and out of the epithelium-lined channel in programmable intervals mimicking true smoking behavior, said Richard Novak, a co-author of the study and a senior staff engineer at the Wyss Institute, who helped design the smoking instrument

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A full knowledge of the lung deposition pattern of PM2.5 and UFPs in a given aerosol is insufficient to assess its inhalation risks, since particles of the same size can have very different physical properties and chemical composition, and thus pose different levels of toxicity and risk (as in the examples examined by Ogulei et al mentioned above)
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