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These isolated miRNAs were reverse transcribed using a TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA) and a TaqMan MicroRNA Assay (Applied Biosystems

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The 2016 revised statement on e-cigarettes by the US Flavoring and Extract Manufacturers Association about these chemicals (which have been safety assessed for use in foods and beverages, but not for inhalation) is further cause for concern: The manufacturers and marketers of e-cigarettes and all other flavored tobacco products, and flavor manufacturers and marketers, should not represent or suggest that the flavor ingredients used in these products are safe because they have FEMA GRASTM status for use in food because such statements are false and misleading