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To accurately characterize macrophage phenotypes, we used a combination of markers: CD14 as a general marker for cells of the monocyte/macrophage lineage, CD86 for M1-like activation, and CD163 and CD206 for M2-like activation

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Alternatives to animal testing include sophisticated tests using human cells and tissues (also known as in vitro methods), organs-on-chips, advanced computer-modeling techniques (often referred to as in silico models), and studies with human volunteers

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The resulting MMG-SEM models are compared to select the optimal number of clusters based on the balance between model fit and parsimony