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Its a Bible Top 40, a Roger McGuinn singalong, but despite this I have never before heard the final verse, and these men, after a lifetime of scrubbing out boxwoods and bloodwoods, lap it up: What do workers gain from their toil

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RT-PCR RNA was extracted from fully matured HBE cells with the AllPrep DNA/RNA Mini kit (80204, QIAGEN AG, Germantown, MD) and treated with DNase I (M0303S, New England BioLabs Inc., Ipswich, MA) before reverse transcription with the iScript cDNA Synthesis Kit (1708891, Bio-Rad)

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For instance, when asked about drinking and driving, most participants mentioned that you could hurt yourself or somebody else