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For our SNP-HSI association testing, we followed the model employed by systemic GWAS analyses for a multitude of phenotypes (see adjusting for the following covariates: sex, age, age 2 , age sex, age 2 sex, and PC eigenvectors, with the age 2 and interaction terms among the age and sex variables intended to account for non-linear associations

Burstyn (2014) estimated the potential levels of exposure to PG from e-cigarettes, assuming extreme consumption of the liquid per day via vaping (5 to 25 ml/day and 5095 percent propylene glycol in the liquid) and concluded that levels of propylene glycol in inhaled air can reach 16 mg/m 3 . With an assumption of complete absorption via inhalation, Burstyn concluded that estimated levels of exposure to propylene glycol and glycerin are close enough to TLV to warrant concern. However, putting these values in perspective with the clinical data from intravenous administration of PG in adults may be useful

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