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Methods Isolation of CD4 + T lymphocyte subsets Peripheral blood mononuclear cells (PBMCs) of healthy donors from the French Blood Bank (Etablissement Franais du Sang, Creteil, France) were separated using UNI-SEP Separation Tubes (Novamed, Jerusalem, Israel), and CD4 + T cells were further enriched by negative selection using a CD4 + T cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany)

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