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Throat hit: The physical sensation at the back of the throat created by the tobacco blend and the temperature of the aerosol

While the rats pressed the lever in hope of getting the sugar reward, they were presented intermittently with the sound cue, to assess the cues ability to trigger excessive pursuit of reward

This is the key feature that differentiates our method from conventional approaches

Nigel Gray, Jack Henningfield, John Seffrin and Witold Zatonski, Tobacco: Science, Policy and Public Health , Oxford University Press, second edition, 2010, 776 pages (ISBN 9780199566655)

Instead, saliva was used as a surrogate matrix, which is widely accepted as reflecting the overall oral microbial community, as it contains microorganisms shed from multiple intraoral niches, including both supragingival and subgingival sites, as we and others have previously demonstrated44