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Transposition mapping for detection of on-target and off-target integration Genomic DNA (2 g) was digested with the XmaI restriction enzyme, heat-inactivated, diluted, and subjected to intramolecular ligation in a 500 l reaction at 1 ng l 1

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Interestingly, ToBRFV MP is less functional for viral cell-to-cell transport than TMV MP (Hak and Spiegelman 2021), suggesting that the possible evolution of ToBRFV via adaptation to Tm-2 2 tomato plants may have been associated with a fitness cost

This should grow, especially as BAT continues to: scientifically substantiates reduced-risk claims for New Category products, leading to advantageous regulation and lower taxation relative to legacy products broaden consumer awareness of the existence and benefits of new products, scale, and enter new markets see shrinking combustible volumes