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The signal was visualized with 100 L of 1 mg/mL p -nitrophenylphosphate (Roth) in substrate buffer (50 mM Tris-HCl, 150 mM NaCl, 2 mM MgCl 2 , pH 9.6)

[84] Long-term nicotine use affects a broad range of genes associated with neurotransmission, signal transduction, and synaptic architecture

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As discussed above, it is now recognized that many patients with COPD are people who never smoked, but there is relatively little data on the pathological changes seen in these patients

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