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Healthy untreated cells were considered as negative control for the assay, whereas cells treated with 1% Triton X-100 were considered as the positive control for maximum LDH release

Most compounds of concern were detected at 0.3 to 70 g per puff

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14 Moreover, Cavazos-Rehg et al found that smoking cessation was associated with a decreased risk of persistent or recurrent mood disorder in adults with a prior history of a mood or anxiety disorder, 13 similar to findings by Blanco et al which found that smoking remission was not associated with increased risk of psychiatric disorders among adults with existing mood, anxiety, or substance abuse disorder