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Methods Long-term CSE exposed human bronchial epithelial cell culture BEAS-2B cells were grown in RPMI 1640 (Lonza, Walkersville, MD) supplemented with 15% heat-inactivated Fetal Bovine Serum (FBS), penicillin (100 U/ml) (Lonza) and streptomycin (100 g/ml) (Lonza) on collagen-coated plates

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Struggling with nicotine withdrawal as all around you give in to temptation, whether it be cigarettes, alcohol or food (or all three), is hard, but learning to first recognise and then successfully deal with whatever triggers your nicotine cravings can be a significant step towards quitting smoking for good