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d - f , HFHCD-fed Prkaa1 fl/fl and Prkaa1 IE mice (SPF) were treated with nicotine water for 2 weeks, and organoids were then isolated and infected with LV (lentivirus)-Ctrl or LV-Smpd3, the infected organoids were plated and cultured for 7 days and treated with nicotine (1 g ml 1 ) for the last 3 days before the detection of ceramide production and secretion

It has been suggested that pre-conception exposure to tobacco, chemicals, and stress can cause epigenomic changes in germ cells, adversely affecting the health of the next generation 36

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Research on the effects of e-cigarette use faces challenges due to these variations

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