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Immune cell isolation from induced sputum and peripheral blood To eliminate mucus from sputum samples, the same volume of 0.1% dithiothreitol (Sigma, MO, USA) was added, the tube was shaken for 20 min at 37 C, and the mixture was filtered through a 70 m strainer

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Publix stated that there were no slip and falls at the subject store in the three years before her accident

That heavy promotion was curious, as three years earlier, PMIs chief executive had made a claim so surprising it had made headlines all over the world