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The tobacco should be properly harvested at maturity, free from excessive damage, and properly handled to maintain leaf integrity

A smoker who drops from 20 cigarettes per day down to 15 may not see meaningful declines in carcinogen exposure, Conley said

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Research shows that nicotine in e-cigarettes increases the infiltration of inflammatory cells, including eosinophils, from the blood into the airways, stimulates the production of type 2 inflammatory cytokines such as IL-4, IL-5, and IL-13, and promotes mucin secretion, exacerbating airway inflammation and hyperresponsiveness, ultimately worsening asthma [66, 67]

Although the main alkaloid found in tobacco-derived products, including e-liquids, is nicotine, several minor tobacco alkaloids have been identified