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Study on the Blood-Brain Barrier Transport of Ligustilide I and Its Mechanism of Action Against Cerebral Ischemia

Typical murine plasma half-life: 4-8 hours after subcutaneous injection (vs 10-20 minutes for native IGF-1)

When to Be Concerned In a research context, further evaluation may be warranted if observations include: Rapidly expanding redness beyond the injection site Severe or escalating pain Persistent swelling lasting multiple days Signs of infection (increasing warmth, spreading redness, discharge) Systemic symptoms such as generalized hives, shortness of breath, dizziness, or facial swelling Severe or systemic reactions should be treated as medically significant and require appropriate clinical evaluation

Upon stimulation of our Ferroptosis reporter HT-1080 cells using the ferroptosis activator RSL3, the cell membrane ruptures and the reporter protein HMGB1::Lucia is released in the extracellular milieu

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