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London, England: Constable & Robinson

RT-PCR RNA was extracted from fully matured HBE cells with the AllPrep DNA/RNA Mini kit (80204, QIAGEN AG, Germantown, MD) and treated with DNase I (M0303S, New England BioLabs Inc., Ipswich, MA) before reverse transcription with the iScript cDNA Synthesis Kit (1708891, Bio-Rad)

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"Our work suggests that the reality is much more nuanced than the idea of 'feel bad, smoke more.' Specifically, we find that sadness appears to be an especially potent trigger of addictive substance use." Senior co-author Dr