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The underlying mechanism through which the circadian rhythm regulates testosterone secretion may involve the activation of the PKAsteroid acute regulatory protein signaling pathway [71]

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The relationships of the metabolic pathways are illustrated in Fig

Oxidative stress response is clock dependent, as many of the antioxidant enzymes display circadian variations [48,49,50] and BMAL1 plays an important role in their regulation [51]

The Majorbio Cloud Platform ( was used for bioinformatics analysis [21]

These include reduced gonadotropin secretion due to impaired positive estrogen feedback on luteinizing hormone (LH) secretion in women (21), increased adrenal androgen secretion (22), and direct blockade of gonadotropin action at the gonadal level (23, 24)