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Set up proper storage: Ensure you have reliable refrigeration

Prior to exposure, cells were pelleted (250 g, 5 min, room temperature), resuspended (5 10 4 in 0.5 mL), and incubated (30 min, 37 C) in AIM V serum-free medium with serial dilutions of DARA, ISA or control human IgG1 mAb (10 g/mL-100 g/mL)

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For instance, exercise can induce the expression of PGC-1 in skeletal muscles and the heart, or by activating the adenosine 5-monophosphate (AMP)-activated protein kinase (AMPK)/PGC-1 signaling pathway, thereby increasing the number and function of mitochondria, enhancing ATP synthesis capacity, and improving the tolerance of the heart to hypoxia

Int J Prev Med