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All software executed by the pipeline is containerised meaning that no additional installation is required for both local or high-performance computing

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Subcellular localization of YFP-tagged protein and bimolecular fluorescence complementation (BiFC) assay YFP or Split-YFP protein fusion plasmids ( pAM-PAT-GW-YFP or pAM-PAT-YFP-GW for YFP and pAM-PAT-GW-nYFP and pAM-PAT-GW-cYFP for split-YFP) were co-transformed into Arabidopsis protoplasts as described above and then incubated in a 21C growth chamber for 24 h under dark conditions