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Protein purification Harvested bacterial cells were lysed on ice for 60 min by treatment with lysozyme (0.2 mg ml 1 ) and Protease Inhibitor Cocktail Tablets, EDTA-Free (Sigma-Aldrich) followed by sonication 5X 20 s and centrifuged at 30,000 g for 30 min

Finally, it is important to note that NAC has antioxidant properties in addition to being able to provide cysteine for glutathione synthesis

This is particularly important given the variable blood pressure effects different users experience

Accessed February 6, 2012

Inhibition of NGLY1 inactivates the transcription factor Nrf1 and potentiates proteasome inhibitor cytotoxicity