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FIGURE 2 2.1 Forming the basis: antioxidant and metabolic enhancement The most studied antioxidant supplement in stored RBCs is ascorbic acid, with human studies indicating that it attenuates oxidative stress and lesions (Tzounakas et al., 2022a) and boosts antioxidant defenses (Ravikumar and Rajashekaraiah, 2017)

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However, another study using rats showed that protein levels of NAMPT, COX1, and PGC-1a were unchanged in skeletal muscles after 4 weeks of treadmill exercise, and NAD + levels or other mitochondrial readouts were not included 308

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