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Here, we have used a DIA mass spectrometry approach to compare a range of artemisinin resistant (Cam3.II R539T , Cam3.II C580Y and MRA1240) and sensitive parasites (Cam3.II REV and MRA1239) and for the first time also performed comparative proteomics analysis in 3-6 hpi ring-stage parasites, which is the stage at which artemisinin resistant parasites display differential survival relative to sensitive parasites (Supp data sheet 1)

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This finding indicates that GPNA can significantly impede the uptake of Gln by cancer cells, thereby reducing intracellular Gln levels

One tube (EDTA-containing) was centrifuged at 16,000 g for 10 min