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In Vitro Maturation, Fertilization and Preimplantation Embryo Culture For in vitro maturation (IVM) of oocytes, every 50 thawed COCs was sequentially cultured in 500 l MI- and MII-medium in four-well culture plates (Nunc, Roskilde, Denmark) at 38.5C in an atmosphere with 5% CO 2 and saturated humidity for 22 h each

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