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Important caveat: human evidence for DSIP is limited and dated , and decades of study never reached regulatory approval

Human clinical trials for MOTS-c are underway but not yet published in their final forms

Furthermore, CatG participates in complex inflammatory response regulation processes, including cytokine, chemokine, and growth factor processing and activation, as well as apoptosis induction through protease three pathways ( In pathological states such as bronchiectasis and COPD, NSP activity in sputum and bronchoalveolar lavage fluid increases markedly, with activity levels correlating positively with disease severity, acute exacerbation risk, and lung function decline rates ( 2.3 Role of DPP1 in NSP activation DPP1 plays an essential role in activating NSP precursor proteins during neutrophil maturation

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Animal studies indicate dose-dependent effects, with toxic doses possibly affecting copper metabolism or interacting with embryonic development in hypersensitive models, warranting caution during pregnancy