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The breakdown process of BCAAs is similar in all species, initially forming branched-chain -keto acids (BCKAs) via branched-chain amino acid transferase (BCATs) and transferring nitrogen to nitrogen receptors (the most common nitrogen receptor is -ketoglutaric acid (-KG) to form glutamate)

the NF-kB pathway) independent of ligand engagement (121123)

doi: 10.1371/journal.pbio.2006203 17 FengSTangDWangYLiXBaoHTangCet al

While cystine is generally considered the rate-limiting amino acid for GSH synthesis [47], glutamate also plays a crucial role in GSH production, especially in cancer cells with a high demand for GSH

Induced cultivations were kept on separate incubator from uninduced ones to avoid any effect of the highly volatile cyclohexanone