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Importantly, women appear to respond well at lower injectable doses, and the most common approach combines topical and injectable use simultaneously for maximum cosmetic effect

Visible changes usually appear in 4 to 6 weeks

Imura, Y., Nishida, M., Ogawa, Y., Takakura, Y., and Matsuzaki, K., Biochim

Cell lines were cultured in the recommended medium and condition (DMEM medium with 10% FBS and 1% antibiotics (penicillin-streptomycin 10,000 U mL 1 ) at 37 C in a humidified atmosphere containing 5% CO 2 )