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The raw milk was spun at 3565 g to remove excess fat and the remaining milk was adjusted to 340 mM NaCl with 0.1 mM phenylmethylsulfonyl fluoride (PMSF)

58 During the progression from preneoplastic glycogenotic to glycogen-poor neoplastic cell populations, this process is linked to an isoenzyme shift from the pyruvate kinase characteristic of normal hepatocytes (PK-M 1 ) to PK-M 2 , 45,59 which is critical for regulating the glycogen shunt flux

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Each intake period consisted of four weeks, with a two-week washout period between condition assignments