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Using MAGeCKflute CutoffCalling, we compared the gene hits by categorysensitizers (those whereby a loss sensitizes to 5FU) and resisters (those whose loss promote insensitivity to 5FU)and we observed several metabolic genes in both (Fig

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The Glutathione Sepharose gel was then washed with 100 times the volume of ice-cold PBS, and then incubated with 10 U/mL thrombin (dissolved in PBS, freshly prepared) at 4 C for 8 h to cleave the GST tag from GK, releasing the GK from the Glutathione Sepharose gel

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