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Inhibiting glutamine with the glutamine antagonist 6-diazo-5-oxo-L-norleucine (DON) in the culture medium enables CAR-T cells to have enhanced mitochondrial OXPHOS and reduced glycolytic activity while preserving more Tn or Tcm subpopulations (152, 153)

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Cwiklowska K, Westhoff MA, Freisinger S, Dwucet A, Halatsch ME, Knippschild U, Debatin KM, Schirmbeck R, Winiarski L, Oleksyszyn J, Wirtz CR, Burster T (2018) Viability of glioblastoma stem cells is effectively reduced by diisothiocyanate-derived mercapturic acids

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