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Those with more body fat can use 90 degrees (straight in), while leaner individuals may prefer 45 degrees to ensure the injection stays in the subcutaneous layer

The selected temperature was well above the thermoneutral zone to ensure exposure to heat stress, while the 4-hour duration was sub-lethal, sufficient to induce chronic stress without causing acute mortality

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Consistently, ID also causes atrophy of the thymus, the primary site of T cell maturation (93)