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Thus, we investigated mutants that result in defective respiratory chain complexes ( nde1 , nde2 , rip1 and cox17 ), chemical treatments to inhibit complexes of the respiratory chain (antimycin A (AntA), potassium cyanide (KCN) to inhibit complexes III and IV, respectively, and paraquat to induce mitochondrial oxidative stress), mutants of pathways counteracting ROS ( ccp1 , sod1 , sod2 ), and strains that express Mia40 and Erv1 under the control of regulatable promoters (Figure 5D and E