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Freeze-dried biomass was homogenized in a standard potassium phosphate extraction buffer (pH 7.0) supplemented with stabilizers and protease inhibitors

Cell culture and characterization The human BBB co-culture model based on stem cell-derived endothelial cells (hEC) and brain pericytes (PC) were established and characterized by the group of Ceccelli et al

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The data revealed associations with fasting C-peptide/glucose changes and differences between the UFMs and ND youth for a panel of these proteins

These bacteria were identified as Bacillus megaterium (Bm), Enterobacter sp