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Measurement of the concentration of macro and micro elements The wet digestion method was used to measure the concentration of macro and microelements 27 for which 500 mg of dry and ground leaf samples were digested in a mixture of sulfuric acid (H 2 SO 4 ) and nitric acid (HNO 3 ) at a ratio of 1:5 (v/v) at 60 C for 24 h

Here, we elucidated two relevant metabolic behaviors to alleviate energy limitations by proposing new hypotheses

In particular, miR-122-5p, which is already established as a critical hepatic biomarker, was upregulated in MASLD serum, consistent with studies in adults

Blots were then incubated with secondary antibody for one hour at room temperature

Early formulations, such as Tyrodes and Lockes solutions, provide only basic ionic balance and are insufficient for long-term cell maintenance (Men, 2024)