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It is greater in normal cattle than in other normal domestic animals

10.1016/j.jep.2021.114667 17 ChenL.HuangJ.JiY.ZhangX.WangP.DengK.et al (2016b)

Immunoblotting Proteins were extracted from cultured SCD erythroblasts or spleen Ter119+ erythroblasts with modified RIPA buffer (150 mM NaCl, 50 mM Tris, pH 7.5, 1% NP40, 0.25% Na-deoxycholate) plus protease inhibitor cocktail (Sigma) and stored at 80 C up to processing