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S2CID 205563521

Subsequently, 200 L media containing 143B cells at a density of 210 3 was added to each well

Specifically, the ROS scavenger butylated hydroxyanisole delayed TNF-induced necroptosis but had no effect on necroptosis induced by direct RIPK3 oligomerization, suggesting that ROS may facilitate upstream signaling events rather than serve as an obligate execution mechanism 80