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Primers used in 5 to 3 direction: sVEGFR1 forward:AGAGGTGAGCACTGCAACAA, sVEGFR1 reverse: TCTCCTCCGAGCCTGAAAGT, UBC forward: AGTAGTCCCTTCTCGGCGAT and UBC reverse: GCATTGTCAAGTGACGATCACAG

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