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Recombinant Kaposis sarcoma-associated herpesvirus (rKSHV.219 51 ) was obtained from BrK.219 cells 72 (generously provided by Thomas Schulz, Hannover Medical School, Hannover, Germany) treated with Roswell Park Memorial Institute (RPMI) 1640 medium (Life Technologies) containing 0.625 g/ml anti-human IgM (-chain-specific, Southern Biotech), 0.05 g/ml 12-O-tetradecanoylphorbol-13-acetate and 8% FBS