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To measure the CRT expressions, after treatment, the cells were washed with cold PBS, and further incubated with anti-CRT polyclonal antibody (diluted 1:100, anti-human antibody for G7, WL1, U87, and U251 cells and anti-mouse antibody for GL261 and G422 cells) at 4 C for 30 min, and stained with Alexa Fluor 647 labeled goat antirabbit IgG (H&L), a highly cross-adsorbed secondary antibody (1:200 dilution), for 30 min at room temperature

Mescher M, Merkle H, Kirsch J, Garwood M, Gruetter R (1998) Simultaneous in vivo spectral editing and water suppression

(10) While not a substitute for food safety practices, garlic enhances protection against harmful microbes

This B12-intrinsic factor complex travels to the ileum (the last section of the small intestine), where it is absorbed into the bloodstream