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The N-terminal tyrosine modification provides enhanced stability vs the unmodified HGH Fragment 176-191

Using ten online computational tools, the study revealed four nsSNPs among the 17 nsSNPs identified as most deleterious and disease associated

Protein concentrations were determined by measuring the absorbance at 280 nm, A 280 , with calculated extinction coefficients as provided by the ProtParam server ( according to the formula 280 = 1490 n Tyr + 5500 n Trp + 125 n Cystine M 1 cm 1 , plus a further increment of 7600 M 1 cm 1 to account for the contribution of the Pap side chain in this spectral region, if applicable

The system presented an LOD of 4 nM with a linear operating range of 6300 nM

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