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The sample was dialyzed against TE buffer (10 mM Tris, 1 mM EDTA, pH 8.0) containing decreasing concentrations of NaCl at 1.2, 1, 0.8, and 0.6 M, each for 2 h at 4 C, followed by dialysis against TE overnight at 4 C

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10.1007/s11248-007-9098-7 189 WyciskK.KimE

Naylor JC, Kilts JD, Hulette CM, Steffens DC, Blazer DG, Ervin JF, Strauss JL, Allen TB, Massing MW, Payne VM, Youssef NA, Shampine LJ, Marx CE

The samples were then washed with precooled anhydrous acetone, embedded in graded HM20 resin (33%, 66%, and 100%), and polymerized under UV light for 18 h at 45 C