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During both procedures, tail-blood glucose was measured at 0, 15, 30, 45, 60, 90, and 120 minutes with a Contour blood glucose meter (Ascensia Diabetes Care, Parsippany, NJ)

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Considering the pharmacokinetics of human growth hormone (hGH), the common practice of administering subcutaneous human growth hormone (hGH) injections in the evening between 20:00 and 21:00 leads to a peak in hormone levels approximately 46 hours later, aligning closely with the natural nocturnal surge that typically occurs around midnight to 02:00 ( Switching to morning GH injections would indeed alter this pattern, causing higher circulating GH levels during daytime hours than under normal physiological conditions

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