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Typically, cells were seeded in 100 L RPMI phenol red free media per well in duplicates on a black 96-well plate with clear bottom (Thermo Scientific)

10.1016/j.jep.2011.04.072 49 KimK.HuhY.LimK.-M

The PVC microplastics (28 0.5 mg per pellet) were spherical with a radius of 2 mm and contained 37.8 wt% of DEHP (C 24 H 38 O 4 ) 65 determined in the laboratory in our previous study 27